Endosymbiotic alga from green hydra under the influence of cinoxacin.

Cinoxacin (Cxn) showed a strong effect on the endosymbiotic alga Chlorella; it was significantly damaged. Changes in algal color, position, structure and ultrastructure were found. In some algal cells ultrastructures were completely destroyed. The antichloroplastal and antimitochondrial effect was especially expressed. Damage to the thylakoid system of chloroplasts was more pronounced with increasing Cxn concentration. Some of the mitochondria were swollen and some of them were completely destroyed. From the evolutionary point of view, the correlation between antibacterial, and antichloroplastal and antimitochondrial effect of Cxn points to the evolutionary connection of chloroplasts and mitochondria with eubacteria.

Formation of vesicles in diluted aqueous solutions of surfactant investigated by direct analysis of light scattering.

Formation of aggregates in the binary systems of double-tailed surfactant, sodium 4-(1-pentylheptyl)benzenesulfonate, and water in the dilute regime was supposed to occur within 5.0-8.5% of surfactant concentration. The size of particles was determined by light scattering. In addition, the samples were observed at room temperature using an Axiovert 35 Zeiss polarized light microscope operated with differential interference contrast optics. The observed aggregates could, in theory, belong a vesicle phase. All the histograms obtained by light scattering showed a bimodal distribution of particles. Weight factors including intensity, volume and number distribution indicate 97-100% of small aggregate sizes, since the peaks for the big sizes indicate only a small number of the aggregate population. Small aggregates have shown monodispersity with diameters of the aqueous core amounting to 38.94 and 54.94 nm relating to the surfactant concentration of 6.0 and 8.0%, respectively. Hydrodynamic radii determined by the Cumulant method, by the inverse Laplace transformation, and using a plot 1/tau vs. q2, showed values within the usual precision limits.

Chromoplasts--the last stages in plastid development.

The results of investigations on the development of chromoplast fine structures in various plants are reviewed. Emphasis is placed on the specific pigment-containing structures and their development during chromoplast formation. There is a large variety of these structures, although four fundamental types can be discerned. These are plastoglobules, membranes, crystals, and tubules. During chromoplast development, various types of structure follow one after the other, or they may even be present simultaneously in the same chromoplast. Depending on the structures present in chromoplasts their pigment content also varies. It is still not clear whether the type of structure defines the pigment content of the chromoplast or vice-versa. Various possible ways of chromoplast development and dedifferentiation are discussed.

Transformation of plastids in the leaves of Acer negundo L. var. odessanum (H. Rothe).

The leaves of Acer negundo L. var. odessanum (H. Rothe), if permanently exposed to strong sunlight, do not green, but remain yellow and finally become bleached. In yellow leaves the plastids contain single thylakoids and no grana. In plastids of bleached leaves, however, only vesicles are present. The concentration of chlorophylls and photosynthetic activity are much lower in those leaves than in the green ones. If the illumination is reduced (e.g. by shading) both the yellow and the bleached leaves become greenish, and even fully green after a few days at a sufficiently low light intensity. The plastids of yellow-green leaves contain small grana. In dark green leaves the thylakoid system of the chloroplasts is normally developed forming true grana, regardless of whether the leaves were originally green, or became green by shading the yellow or bleached ones. Their pigment concentration and photosynthetic activity are also normal. If green leaves are exposed to sunlight they do not yellow or bleach. During a 3-week period the structure of the thylakoid system did not perceivably change, with the exception that large plastoglobules formed in the stroma.